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Shukla, S. K.
- Simultaneous Determination of Saccharin and Aspartame in Drinks by UV-Spectrophotometry
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Authors
Affiliations
1 Central Forensic Science Laboratory, Directorate of Forensic Science, Ministry of Home Affairs, Govt. of India, Ramanthapur, Hyderabad, IN
2 Central Forensic Science Laboratory, Directorate of Forensic Science, Ministry of Home Affairs, Govt. of India, Chandigarh, IN
3 Forensic Science Unit, Department of Chemistry, Osmania University, Hyderabad, IN
1 Central Forensic Science Laboratory, Directorate of Forensic Science, Ministry of Home Affairs, Govt. of India, Ramanthapur, Hyderabad, IN
2 Central Forensic Science Laboratory, Directorate of Forensic Science, Ministry of Home Affairs, Govt. of India, Chandigarh, IN
3 Forensic Science Unit, Department of Chemistry, Osmania University, Hyderabad, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 5 (2011), Pagination: 737-740Abstract
A simple and relatively less expensive UV spectrophotometric method was developed for simultaneous determination of saccharin and aspartame in soft drinks by multicomponent analysis method. Wavelengths of 235 nm and 257 nm were selected for determination of saccharin and aspartame respectively. The limit of detection for saccharin and aspartame was found to be 0.0015 g L-1 and 0.04 g L-1 respectively. The method obeys beers law within the concentration range of 0.002 g L-1 to 0.04 g L-1 and 0.06 g L-1 to 0.28 g L-1 for saccharin and aspartame respectively. This method was found to have the coefficient correlation (r2) value of 0.9997 and 0.999 for saccharin and aspartame respectively. The method was validated for accuracy, precision and repeatability. The proposed method was found to be simple, accurate, reproducible, sensitive, free form interferences, inexpensive and having good applicability to the routine analysis of samples of foods and beverages.Keywords
Saccharin, Aspartame, Blend, Multiple-Component, Drinks.- Determination of Clonazepam in Chocolate using High Performance Liquid Chromatography and Further Confirmation by Gas Chromatography-Mass Spectrometry
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Authors
Affiliations
1 Central Forensic Science Laboratory, Govt. of India, Hyderabad - 500 013, IN
2 Central Forensic Science Laboratory, Govt. of India, Chandigarh - 160 036, IN
1 Central Forensic Science Laboratory, Govt. of India, Hyderabad - 500 013, IN
2 Central Forensic Science Laboratory, Govt. of India, Chandigarh - 160 036, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 5 (2011), Pagination: 761-765Abstract
Clonazepam, a nitrobenzodiazepine and a scheduled drug is one of the highly abused drugs in recent times. An analytical methodology was developed for the detection and quantitation of clonazepam in chocolate sample. The method involves the extraction of the chocolate sample by solvent extraction with chloroform under basic conditions (pH: 8-9). The extract was used for the GC-MS analysis in SIM and full scan modes for the detection of the analyte present. For quantification, the extract was re-dissolved in the mobile phase, Acetonitrile: 40mM potassium di hydrogen-phosphate (6:4,v/v), pH adjusted to 3.9. The separation was achieved on a Phenomenex Gemini C18 HPLC reversed-phase column (150mmx4.6mmx5μ) with the mobile phase and diode array detection with UV range 200-370 nm. The calibration plot for the determination of clonazepam is based on linear regression analysis (y=51.405x + 84.924; r2=0.9989). The limit of detection of clonazepam was estimated as 0.1μg/ml with a signal-to-noise ratio of 3:1 and limit of quantitation of clonazepam was estimated as 0.35μg/ml with a signal-to-noise ratio of 10:1. The clonazepam detected in the chocolate was found to be 0.0763μg/mg of the chocolate. The precision studies given a % RSD range of 0.28-3.9 % with respect to peak area and 0.51-1.03 % with respect to retention time. The method is robust, specific and reliable for the qualitative and quantitative analysis of clonazepam in chocolate and the law enforcement laboratories can utilise the method for routine analysis.Keywords
Clonazepam, Drug Abuse, Chocolate, GC-MS, HPLC.- Interference Study on Artificial Sweeteners in Forensic Toxicological Screening by TLC
Abstract Views :196 |
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Authors
Affiliations
1 Central Forensic Science Laboratory, Directorate of Forensic Science, Ministry of Home Affairs, Govt. of India, Ramanthapur, Hyderabad – 500 013, IN
2 Central Forensic Science Laboratory, Directorate of Forensic Science, Ministry of Home Affairs, Govt. of India, Chandigarh, IN
3 Forensic science Unit, Department of Chemistry, University College of Science, Osmania University, Hyderabad – 500 007, IN
1 Central Forensic Science Laboratory, Directorate of Forensic Science, Ministry of Home Affairs, Govt. of India, Ramanthapur, Hyderabad – 500 013, IN
2 Central Forensic Science Laboratory, Directorate of Forensic Science, Ministry of Home Affairs, Govt. of India, Chandigarh, IN
3 Forensic science Unit, Department of Chemistry, University College of Science, Osmania University, Hyderabad – 500 007, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 3 (2011), Pagination: 419-422Abstract
A study was carried out to assess the possible interference of artificial sweeteners (Saccharin, sucralose, aspartame and cyclamate) while screening for drugs and pesticides in stomach content, urine and tissues by TLC for forensic toxicological purposes. Even though these sweeteners were extractable in acidic drugs extract and pesticide extract but they did not interfere in the analysis of drugs and pesticides. Therefore the screening methods usually used in forensic toxicological analysis for drugs and pesticides can be applied as such without any modification as the artificial sweeteners were not found to interfere.